Glycosylation is a prevalent, yet heterogeneous modification with a broad range of
implications in molecular biology. This heterogeneity precludes enrichment strategies that
can be universally beneficial for all glycan classes. Thus, choice of enrichment strategy has
profound implications on experimental outcomes. Here we review common enrichment
strategies used in modern mass spectrometry–based glycoproteomic experiments, including
lectins and other affinity chromatographies, hydrophilic interaction chromatography and its …